Metabolite analyses of single cells

Single-cell analyses of circulating tumor cells

Circulating tumor cells (CTCs) are a population of tumor cells mediating metastasis, which results in most of the cancer related deaths. The number of CTCs in the peripheral blood of patients is rare, and many platforms have been launched for detection and enrichment of CTCs. Enumeration of CTCs has already been used as a prognosis marker predicting the survival rate of cancer patients. Yet CTC...

Integrated ‘Omics’, Targeted Metabolite and Single-cell Analyses of Arctic Snow Algae Functionality and Adaptability

Snow algae are poly-extremophilic microalgae and important primary colonizers and producers on glaciers and snow fields. Depending on their pigmentation they cause green or red mass blooms during the melt season. This decreases surface albedo and thus further enhances snow and ice melting. Although the phenomenon of snow algal blooms has been known for a long time, large aspects of their physio...

A Yeast Metabolite Extraction Protocol Optimised for Time-Series Analyses

There is an increasing call for the absolute quantification of time-resolved metabolite data. However, a number of technical issues exist, such as metabolites being modified/degraded either chemically or enzymatically during the extraction process. Additionally, capillary electrophoresis mass spectrometry (CE-MS) is incompatible with high salt concentrations often used in extraction protocols. ...

Observation of subcellular metabolite gradients in single cells by laser ablation electrospray ionization mass spectrometry.

Single-Trial Analyses: Why Bother?

with subjects’ judgment of attentional state (see also VanRullen et al., 2011 for a review of evidence linking alpha oscillations to perception and attention). Multivariate methods are often used to characterize the spatial–temporal variance in each trial in order to derive pattern classifiers (see however Friston et al., 1996 for a more traditional use). For instance, Touryan et al. (2011) use...